ارزیابی تحمل به خشکی در لاین‌های اینبرد نوترکیب برنج با استفاده از شاخص‌‌های تحمل به تنش

نویسندگان

1 دانش‌آموخته دکتری، گروه اصلاح نباتات و بیوتکنولوژی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.

2 دانشیار، گروه اصلاح نباتات و بیوتکنولوژی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.

3 دانشیار، گروه تولیدات گیاهی، دانشکده کشاورزی و منابع طبیعی ، دانشگاه گنبد کاووس، گنبد کاووس، ایران.

4 استادیار، گروه اصلاح نباتات و بیوتکنولوژی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.

5 استادیار، پژوهشکده کشاورزی هسته‌ای، پژوهشگاه علوم و فنون هسته‌ای، کرج، ایران.

چکیده

به‌منظور ارزیابی لاین‌های اینبرد نوترکیب برنج حاصل از تلاقی دو رقم برنج اهلمی طارم و سپیدرودبه ترتیب متحمل و حساس به تنش خشکی، آزمایشی در بهار سال 1395 در مزرعه تحقیقاتی دانشگاه گنبد کاووس اجرا گردید. یکصد و شانزده لاین اینبرد نوترکیب در قالب طرح بلوک‌های کامل تصادفی با سه تکرار در دو محیط (بدون تنش و تنش خشکی) بررسی و ارزیابی شد. شاخص‌های MP، GMP، STI، RDY، HM،K1STI و K2STI با عملکرد دانه در هر دو محیط (تنش و بدون تنش) همبستگی بالایی داشتند، بنابراین به‌عنوان شاخص‌های مناسب جهت انتخاب لاین‌های متحمل به تنش خشکی شناسائی شدند. تجزیه بای‌پلات بر اساس دو مولفه اول نشان داد که لاین‌های شماره 12، 112 و 113 به‌عنوان ژنوتیپ‌های متحمل و لاین‌های شماره 43، 50، 64 و 89 به‌عنوان ژنوتیپ‌های حساس شناخته شدند. بای‌پلات شاخص‌های SNPI و SSPI را نیز به‌عنوان معیارهای شناسایی ژنوتیپ‌های متحمل به تنش خشکی معرفی کرد. بر اساس 17 شاخص تحمل و حساسیت به خشکی و عملکرد دانه در شرایط تنش و بدون تنش گروه‌بندی لاین‌ها با استفاده از تجزیه خوشه‌ای به روش WARD انجام گرفت و چهار گروه تشکیل شد. خوشه‌های دوم و چهارم به‌ترتیب با بیشترین و کمترین مقدار K2STI به‌ترتیب معرف ژنوتیپ‌های متحمل‌ و حساس بودند. در مجموع بر اساس تجزیه بای‌پلات و تجزیه خوشه‌ای لاین‌های شماره 112 و 64 به ترتیب به عنوان متحمل‌ترین و حساس‌ترین ژنوتیپ‌های مورد مطالعه در تحقیق حاضر شناسایی شدند. عملکرد دانه لاین شماره 112 در شرایط تنش و بدون تنش به‌ترتیب 5190 و 6640 کیلوگرم در هکتار و عملکرد دانه لاین شماره 64 در شرایط تنش و بدون تنش به‌ترتیب 1640 و 2240 کیلوگرم در هکتار بود.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of Drought Tolerance in Rice Recombinant Inbred Lines Using Stress Tolerance Indices

نویسندگان [English]

  • B. Kazerani 1
  • S. Navabpour 2
  • H. Sabouri 3
  • S. S. Ramezanpour 2
  • Kh. Zaynali Nezhad 4
  • A. Eskandari 5
1 Ph. D. Graduate, Department of Plant Breeding and Biotechnology, Faculty of Plant Production, Gorgan University of Agricultural and Natural Resources Sciences, Gorgan, Iran
2 Associate Professor, Department of Plant Breeding and Biotechnology, Faculty of Plant Production, Gorgan University of Agricultural and Natural Resources Sciences, Gorgan, Iran.
3 Associate Professor, Department of Plant Production, Faculty of Agriculture and Natural Resources, Gonbad Kavous University, Gonbad Kavous, Iran.
4 Assistant Professor, Department of Plant Breeding and Biotechnology, Faculty of Plant Production, Gorgan University of Agricultural and Natural Resources Sciences, Gorgan, Iran.
5 Assistant Professor, Nuclear Agriculture Group, Nuclear Science and Technology Research Institute, Karaj, Iran.
چکیده [English]

To evaluate drought tolerance in rice recombinant inbred lines obtained from a cross between two cultivars of Ahlami-Tarom (tolerant) and Sepidrood (susceptible), a field experiment was conducted in spring 2016 in the research farm of Gonbad Kavous University. One hundered sixteen genotypes were studied using randomized complete block design with three replications in two environments (drought stress and non-stress conditions). MP, GMP, STI, RDY, HM, K1STI and K2STI indices had high correlations with grain yield under the two environments (drought stress and non-stress), therefore were identified as suitable indices for selecting drought-tolerant lines. A biplot analysis perfermed based on the first and second components of principle component analysis showed that lines No. 12, 112, and 113 were the most tolerant, and lines No. 43, 50, 64, and 89 were sensitive genotypes. Moreover, biplot also identified SNPI and SSPI suitable for identifying tolerant genotypes to drought stress. Grouping of lines based on 17 drought tolerantce indices and grain yield in the stress and non-stress conditions was performed by cluster analysis using Ward method, and four clusters were formed. The second and fourth clusters, with the highest and lowest K2STI, included the most tolerant and most sensitive genotypes to drought stress, respectively. Overall, according to the biplot and cluster analyses, lines No. 112 and 64 were identified as the most tolerant and most sensitive genotypes, respectively. Grain yield of line No. 112, in the drought stress and non-stress conditions was 5190 and 6640 kgha-1, respectively. However, grain yield of line No. 64, in the drought stress and non-stress conditions were 1640 and 2240 kgha-1, respectively.

کلیدواژه‌ها [English]

  • rice
  • Genetic diversity
  • Drought Stress
  • cluster analysis
  • discriminant function analysis

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